Goat anti-Rabbit IgG (H+L) Cross-Adsorbed, Alexa Fluor™ 647 (A-21244) (2024)

  • Secondary Antibodies
  • Goat anti-Rabbit IgG Secondary Antibodies

Invitrogen

26 Published Figures

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Goat anti-Rabbit IgG (H+L) Cross-Adsorbed, Alexa Fluor™ 647 (A-21244) (1)

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Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody (A-21244) in ICC/IF

Immunofluorescence analysis of Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor® 647 conjugate was performed using HeLa cells stained with alpha Tubulin Rabbit Polyclonal Antibody (Product # PA5-16891). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/mL primary an... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more

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Product Details

A-21244

Applications

Tested Dilution

Immunocytochemistry(ICC/IF)

4 µg/mL

Flow Cytometry(Flow)

1-10 µg/mL

Product Specifications

Species Reactivity

Rabbit

Host/Isotype

Goat/ IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Gamma Immunoglobins Heavy and Light chains

Conjugate

Alexa Fluor™ 647

  • Alexa Fluor 350
  • Alexa Fluor 488
  • Alexa Fluor 546
  • Alexa Fluor 555
  • Alexa Fluor 680
  • Alexa Fluor 790
  • AP
  • Biotin
  • Cyanine3
  • Cyanine5
  • FITC
  • HRP
  • PE
  • Rhodamine
  • Rhodamine Red-X
  • Texas Red
  • Texas Red-X
  • Request custom conjugation

Excitation/Emission Max

650/671 nmView spectra

Form

liquid

Concentration

2 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.5

Contains

5mM sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient

RRID

AB_2535812

Target

IgG

Cross Adsorption

Against human IgG, human serum, mouse IgG, mouse serum and bovine serum

Antibody Form

Whole Antibody

Product Specific Information

To minimize cross-reactivity, these goat anti-rabbit IgG (H+L) whole secondary antibodies have been affinity purified and cross-adsorbed against human IgG, human serum, mouse IgG, mouse serum, and bovine serum. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there are may be the presence of endogenous immunoglobulins.

Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 647 dye is a near-infrared-fluorescent dye with excitation ideally suited to the 647 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 647 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 647 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. The degree of labeling for each conjugate is typically 2-8 fluorophore molecules per IgG molecule; the exact degree of labeling is indicated on the certificate of analysis for each product lot.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. For the fluorophore-labeled antibodies a final concentration of 1-10 µg/mL should be satisfactory for most immunohistochemistry and flow cytometry applications.

Product will be shipped at Room Temperature.

Target Information

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Goat anti-Rabbit IgG (H+L) Cross-Adsorbed, Alexa Fluor™ 647 (A-21244) (2024)
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